Establishment of tetra-primer ARMS-PCR technique for detecting exo-E415G SNP in Plasmodium falciparum isolatesfrom Central Highlands of Vietnam

Drug resistance in Plasmodium falciparum parasites is associated with the single nucleotide polymorphism (SNP) exo-E415G, which considered as a marker to reduce susceptibility artimisinin and leads treatment failure. This study aims establish procedure based on tetra-primer ARMS PCR technique detect SNP exo-E415G malaria patients who experienced failure artemisinin. DNA sample from P. 3D7 isolate was used optimise SNP. The method then applied screen for 123 blood samples artemisinin 3 provinces (Gia Lai, Dak Nong, Lak) Central Highlands of Vietnam. A fragment (749 bp) containing exo-E415GSNP amplified using primer pairs E415G-Fc E415G-Rc. Specific primers wildtype alen (A alen) mutant (G fragments length 316 474 bp, respectively. genotyping results were consistent Sanger sequencing. failed treatment, frequency observed at 78% (96/123). In conclusion, simple established Exo-E415G. prevalence alenExo-E415G